The results indicate that the group II intron (1300 bp) is present in cel5I. Jinqian Baihua She (JBS) (coin-like white-banded snake, Bungarus parvus) is a commonly used high-value traditional Chinese drug derived from the dried body (with the viscera discarded) of many-banded krait, Bungarus multicinctus multicinctus Blyth (Fam. barcoding of common medicinal snakes in China, of the Royal Society B: Biological Sciences, coding animal life: cytochrome c oxidase subunit  diverg, medicinal snakes in medicated liquor of G, ... To identify the medicinal reptiles that exhibit similar morphology interspecifically, DNA barcoding provides a simple but reliable strategy ( Table 2). (Jin Qian) Bai Hua She (Bungarus Parvus) Dispel Wind-Dampness. Site: International - Español - Português - Deutsch - Français - Italiano - हिंदी - Pусский - 한국어 - 日本語 - اللغة العربية - ภาษาไทย - Türk - Nederlands - tiếng Việt - Indonesian - עברית, AliExpress or diagnostic PCR will suce because both of them are rapid, of JBS. [4] Most species of kraits are covered in smooth, glossy scales arranged in bold, striped patterns of alternating black and light-colored areas. BM1 (1), BM2 (2), BM3 (3), BM4 (4), BF (5), NJ (6), DR1 (7), DR2 (8), OM (9), EP (10), SA (11), DA (12), DF (13), ZY (14), XF (15), negative control (16) (water was used as sample), and DNA marker (M) in bp were indicated. In this last case, the monovalent B. fasciatus antivenom is also moderately effective.

Bungarus multicinctus: Bungarus Parvus: Lysimachia christinae (Kraut) Kraut Lysimachiae: Myrmeleon micans: Larve Myrmeleontis: Lonicera japonica (Blume oder Blumenknospe) Flos Lonicerae: Rosa laevigata (Frucht) Fructus Rosae Laevigatae: Schizonepeta tenuifolia (Kraut) Kraut Schizonepetae: Schizonepeta tenuifolia (Blume u. Blumenknospe) Flos Schizonepetae We established polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and diagnostic PCR based on cytochrome C oxidase subunit I (COI) barcodes of Bungarus multicinctus, genuine Jinqian Baihua She (JBS), and adulterant snake species. Kraits are found in tropical Asia, from near Iran, through the Indian subcontinent (including Sri Lanka and Bangladesh) and on to Southeast Asia (including Indonesia and Borneo).[3].

Thus, these two molecular approaches provide practical tools for quick identification of commercial Baitouweng products and can help ensure the safe use of this TCM product. e sample, D could not be identied for origin through its a, tication. This microorganism usually colonize the upper respiratory tract in approximately 20-40% of healthy humans. The genetic distance and NJ tree demonstrated that there were 241 variable sites in these species, and the average (A + T) content of 56.2% was higher than the average (G + C) content of 43.7%. Durch intensivmedizinische Betreuung, vor allem durch künstliche Beatmung, kann die Sterblichkeit erheblich gesenkt werden. In contrast, other salmonids are endangered in their native settings. (Top) Depiction of the region of the 130b chromosome containing cas genes. Based on sequence alignment of B. multicinctus and its adulterants, we found that Cyt b gene is a good molecular genetic marker for the authentication of B. multicinctus. The female lays a clutch of 12 to 14 eggs in piles of leaf litter, and stays with them until they hatch. chinensis. In common with those of all other venomous snakes, the death time and fatality rate resulting from bites of kraits depend on numerous factors, such as the venom yield and the health status of the victim. Alipay

These drugs do the main actions.

Jin Ying Zi Rosae laevigatae fructus Hagebutten chinesische. To authenticate Panax ginseng among ginseng populations, RAPD analysis was carried out using a 20 mer-random primer. Small order 1-100kg, shipping by courier company, such as EMS,DHL,FEDEX,TNT,ETC. Kraits sind am Tag scheu und nicht aggressiv.

Kraits sind mittelgroße bis große Giftnattern, die meisten Arten erreichen Gesamtlängen von 1,0 bis 1,8 m, B. fasciatus auch bis über 2,0 m. Männchen sind deutlich größer als Weibchen. Qian Hu (Peucedani Radix) Cool and Transform Phlegm Heat. Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were clearly distinguished by the naked eye by utilizing a CCP-based assay in a high-throughput manner. The sequences combined with the data from GenBank were analyzed for divergence and building a neighbor-joining(NJ) tree with MEGA 5.0. Data from sequencing confirmed the reliability of diagnostic PCR identification. Pulsatillae radix is a conventional traditional Chinese medicine (TCM) with common name Baitouweng, and has notable effects on inflammation and dysentery. Five species are included in the Global Invasive Species Database: rainbow trout Oncorhynchus mykiss, Atlantic salmon Salmo salar, brown trout Salmo trutta, brook trout Salvelinus fontinalis, and lake trout Salvelinus namaycush. 浙B2-20120091.

Cause of death is usually respiratory failure—suffocation by complete paralysis of the diaphragm. DNA barcoding identification method based on the COI sequence is accurate and can be applied to identify the common medicinal snakes. Moreover, the significant difference in the luxA gene sequence between type strains of V. harveyi and V. campbellii can be utilized in the detection and identification of these two closely related species.

Inverse PCR using circularized HindIII-digested genomic DNA of type strain V. campbellii as template and primers that amplify genes flanking the luxB gene produced a 1,100 bp amplicon containing the 225 bp luxA gene. RFLP-based methods are generally more economical and faster in comparison to metabarcoding ( Teletchea, 2009; ... Total genomic DNA was isolated using the Plant Genomic DNA Kit (Tiangen Biotech Co., China). Bungarus is a genus of venomous elapid snakes, the kraits, found in South and Southeast Asia. Bai Hua She acts to dispel wind, open channels and collaterals, and dispel wind from the skin. Eleven, performed with an initial denaturing at , Evidence-Based Complementary and Alternative M, visualized with ethidium bromide (EB) staining under UV, sequences of  specimens representing  snake species, BOLD system and GenBank. OBJECTIVE: To establish a DNA barcode database of common medicinal snakes in China for species identification and phylogenetic analysis. The similarity coefficients among the DNA of ginseng plants analyzed were low, ranging from 0.197 to 0.491. Detection of the coa gene in Staphylococcus aureus by PCR-RFLP. We are convinced that the sole prospect for a sustainable identification capability lies in the construction of systems that employ DNA sequences as taxon 'barcodes'. This result is consistent with morphological identification and DNA sequence analyses. This may serve as aposematic colouration in its habitat of grassland and scrub jungle. Finally, the method was applied to obtain a global view of the Salmonidae community in Nalón River (north coast of Spain). Kraits are ophiophagous, preying primarily upon other snakes (including venomous varieties) and are cannibalistic, feeding on other kraits. Methods: The genus Bungarus has 15 species.

All rights reserved. The results showed that each sample can be identified as specific snake species, and Z. dhumnades and B. multicinctus as well as their adulterants can be clearly distinguished at the species level [73][74][75]. These affect the ability of neuron endings to properly release the chemical that sends the message to the next neuron. campanella (5-6), Pu. Following envenomation with bungarotoxins, transmitter release is initially blocked (leading to a brief paralysis), followed by a period of massive overexcitation (cramps, tremors, spasms), which finally tails off to paralysis. CONCLUSION: DNA barcoding based on the COI sequences is accurate and reliable and could be applied to identify the common medicinal snakes in our study.

Der Körper ist relativ schlank und im Querschnitt meist annähernd dreieckig, er läuft zum Rücken hin spitz zu. Once at a healthcare facility, support must be provided until the venom is metabolised and the victim can breathe unaided, especially if no species-specific antivenom is available. Objective: Properties Sweet, salty, warm, toxic Channels Entered Liver For example, Zaocys dhumnades and Bungarus multicinctus are two important snakes used in TCM, and their dried bodies without the viscera have been widely applied to treat several disorders including rheumatoid arthritis, stroke, and convulsion [73][74][75], ... Interestingly, several recent studies have used a panel of full-length barcodes and minibarcodes including COI, 12S rRNA, 16S rRNA, and Cytb to analyze various snake specimens and related TMs collected from the wild and markets in China. Häufig erfolgt der Biss auch im Schlaf, da die Tiere nachts häufig in Häuser kommen. The invention belongs to the technical field of Chinese medicaments and the identification on Chinese medicinal materials, and particularly relates to a PCR method capable of specifically identifying bungarus parvus and a specific primer thereof.

certificate and Origin certificate, or as your markets special requirements. The templates were extracted by the DNA purification system.

No signal was detected for wild type genomic DNA lane, in contrary to the native pMTLcel5I vector lane. It derives from the dried body of infant Bungarus multicinctus Blyth and is e ective in dispelling the wind, removing obstruction of the collaterals, and relieving spasm [ ]. Another practice faking JBS is, splitting the body of large genuine snakes in, merely by their morphological characters is ra, e development of molecular biological techniques, provides a new solution for the identica, Our previous studies indicated that COI bar, allows restriction digestion of the sequence between bases,  and .

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